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KMID : 0364219810240010027
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Korean Journal of Zoology
1981 Volume.24 No. 1 p.27 ~ p.37
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Changes in Protein Patterns During Oogenesis and Development of Drosophila melanogaster
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Lee Yang-Rim
Moon Young-Wha
Chung Sook-Yeon
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Abstract
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Protein patterns at various stages of pre-and post-fertilization in Drosophila melanogaster were analyzed using polyacrylamide gel electrophoresis. Ovaries at various times after hatching, embryos, larvae and pupae were collected and used for protein analysis. The protein patterns were found to vary qualitatively and quantitatively during oogenesis as well as during embryogenesis and metamorphosis. Yolk proteins, which were found to be synthesized in fat body and transferred into the oocytes, appeared in three bands in the gel in the early stages, but these proteins seemded to be converged into a single band in the late stage of oogenesis. The protein patterns in the post fertilization stages seemed to be abruptly changed at two different stages; between embryonic and larval stages and between the second and third instar stages. The pattern in the pupal stages was not very much different from that of the third instar.
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